Introduction:

Yanping Long received her graduate and postdoctoral training in plant genetics and molecular biology with the cutting-edge applications in sequencing technologies. The interdisciplinary background of her integrates both “wet lab” and “dry lab” techniques. Based on the emerging single-cell sequencing and three-generation long sequencing technologies, she has led the development of FlsnRNA-seq for single-nucleus full-length sequencing, FLEP-seq for single-molecule full-length nascent RNA analysis, and Pore-C for Arabidopsis thaliana genome multiway interactions and associated methylation modifications. She has published papers as first author (including co-first authors) and co-corresponding authors in high impact journals, including Nature Plants, Nature protocols, PNAS, Genome Biology, Plant Biotechnology Journal and New Phytologist. 

Research Interests：

◆ Single-cell Sequencing

◆ Long-read Sequencing

◆ Gene Expression and Regulation

Professional Experience：

◆ 2023-Present Research Associate Professor Southern University of Science and Technology, Shenzhen, China

◆ 2019-2023 Research Assistant Professor Southern University of Science and Technology, Shenzhen, China

◆ 2016-2019 Postdoctoral Researcher Southern University of Science and Technology, Shenzhen, China Advisor: Dr. Jixian Zhai

◆  2015-2016 Research Scientist The Beijing Genomics Institute, Shenzhen, China

Educational Background：

◆ 2007-2015 Ph.D. in Developmental Biology Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China

Advisor: Dr. Weicai Yang

◆ 2003-2007 B.S. in Biotechnology Northwest Agriculture and Forestry University, Yangling, China

Honors & Awards：

◆ 2022 “Outstanding Young Women Award” Chinese Society for Plant Biology

Selected Publication：

(*co-first authors, #corresponding author)

1. Zhang K.*, Zhao X.*, Zhao Y., Zhang z., Liu Z., Liu Z., Yu Y., Li Z., Ma Y., Dong Y., Pang X., Jin X., Li N., Liu B., Wendel J., Zhai J., Long Y.#, Wang T.#, Gong L.#. (2023) Cell type-specific cytonuclear coevolution in three allopolyploid plant species. PNAS. 120(40):e2310881120.

2. Mo W.*, Shu Y.*, Liu B. Long Y., Li T., Cao X., Deng X. #, Zhai J.# (2023) Single-molecule targeted accessibility and methylation sequencing of centromeres, telomeres and rDNAs in Arabidopsis. Nature Plants. 9, 1439–1450.

3. Liu Z.*, Yang J.*, Long Y.*, Zhang C.*, Wang D., Zhang X., Dong W., Zhao L., Liu C., Zhai J.#, and Wang E.# (2023) Single-nucleus transcriptomes reveal spatiotemporal symbiotic perception and early response in Medicago. Nature Plants. https://doi.org/10.1038/s41477-023-01524-8

4. Liu Z.*, Kong X.*, Long Y.*, Liu S., Zhang H., Jia J., Cui W., Zhang Z., Song X., Qiu L., Zhai J.#, Yan Z.# (2023) Integrated single-nucleus and spatial transcriptomics captures transitional states in soybean nodule maturation. Nature Plants. https://doi.org/10.1038/s41477-023-01387-z.

5. Qin Y., Long Y., Zhai J.# (2022) Genome-wide characterization of nascent RNA processing in plants. Current Opinion in Plant Biology. 69:102294.

6. Jia J.*, Lu W.*, Liu B., Yu Y., Jin X., Shu Y., Long Y., Zhai J.# (2022) An atlas of plant full-length RNA reveals tissue-specific and evolutionarily-conserved regulation of poly(A) tail length. Nature Plants.  8(9):1118-1126.

7. Li Z.*, Long Y.*, Yu Y., Zhang F., Zhang H., Liu Z., Jia J., Mo W., Tian S. Z., Zheng M., Zhai J.# (2022) Pore-C simultaneously captures genome-wide multi-way chromatin interaction and associated DNA methylation status in Arabidopsis. Plant Biotechnology Journal. 20(6):1009-1011.

8. Yu Y.*, Zhang H.*, Long Y., Shu Y., Zhai J.# (2022) Plant Public RNA-seq Database: a comprehensive online database for expression analysis of ~45 000 plant public RNA-Seq libraries. Plant Biotechnology Journal. 20, 806-808. 

9. Long Y.*, Jia J.*, Mo W., Jin X., Zhai J.# (2021) FLEP-seq: simultaneous detection of RNA polymerase II position, splicing status, polyadenylation site and poly(A) tail length at genome-wide scale by single-molecule nascent RNA sequencing. Nature Protocols. 16, 4355-4381.

10. Long Y.*, Liu Z.*, Jia J.*, Mo W., Fang L., Lu D., Liu B., Zhang H., Chen W., Zhai J.# (2021) FlsnRNA-seq: protoplasting-free full-length single-nucleus RNA profiling in plants. Genome Biology. 22, 66.

11. Mo W.*, Liu B.*, Zhang H., Jin X., Lu D., Yu Y., Liu Y., Jia J., Long Y., Deng X., Cao X., Guo H., Zhai J.# (2021) Landscape of transcription termination in Arabidopsis revealed by single-molecule nascent RNA sequencing. Genome Biology. 22(1):322.

12. Hu D.*, Yu Y.*, Wang C.*, Long Y., Liu Y., Feng L., Lu D., Liu B., Jia J., Xia R., Du J., Zhong X., Gong L., Wang K.#, Zhai J.# (2021) Multiplex CRISPR-Cas9 editing of DNA methyltransferases in rice uncovers a class of non-CG methylation specific for GC-rich regions. The Plant Cell. 33, 2950-2964.

13. Jia J.*, Ji R.*, Li Z., Yu Y., Nakano M., Long Y., Feng L., Qin C., Lu D., Zhan J., Xia R., Meyers BC, Liu B.#, Zhai J.# (2020) Soybean DICER-LIKE2 regulates seed coat color via production of primary 22-nucleotide small interfering RNAs from long inverted repeats. The Plant Cell. 32, 3662-3673.

14. Jia J.*, Long Y.*, Zhang H., Li Z., Liu Z., Zhao Y., Lu D., Jin X., Deng X., Xia R., Cao X., Zhai J.# (2020) Post-transcriptional splicing of nascent RNA contributes to widespread intron retention in plants. Nature Plants 6, 780-788.

15. Long Y.-P.*, Xie D.-J.*, Zhao Y.-Y., Shi D.-Q.#, and Yang W.-C.# (2019) BICELLULAR POLLEN 1 is a modulator of DNA replication and pollen development in Arabidopsis. New Phytologist. doi: 10.1111/nph.15610.

16. Zhang Y.*, Harris C.J.*, Liu Q.*, Liu W., Ausin I., Long Y., Xiao L., Feng L., Chen X., Xie Y., Chen X., Zhan L., Feng S., Li J., Wang H.#, Zhai J.# (2018) Large-scale comparative epigenomics reveals hierarchical regulation of non-CG methylation in Arabidopsis. PNAS. 115, E1069.

17. Fang H., Liu Z., Long Y., Liang Y., Jin Z., Zhang L., Liu D., Li H., Zhai J.# and Pei Y.# (2017) The Ca2+/calmodulin2- binding transcription factor TGA3 elevates LCD expression and H2S production to bolster Cr6+ tolerance in Arabidopsis. The Plant Journal. 91, 1038-1050.

18. Kuo H.Y., Jacobsen E.L., Long Y., Chen X., and Zhai J.# (2017) Characteristics and processing of Pol IV-dependent transcripts in Arabidopsis. Journal of Genetics and Genomics. 44, 3-6.

19. Wang, S.-Q., Shi, D.-Q., Long, Y.-P., Liu, J., and Yang, W.-C.# (2012) GAMETOPHYTE DEFECTIVE 1, a putative subunit of RNases P/MRP, is essential for female gametogenesis and male competence in Arabidopsis. PloS one. 7, e33595.

Patents

1. Zhai J., Long Y., Xiao L., Zhang F., Lu D., Liu Z. A new library construction method for single-cell genome sequencing. ZL2019113118042.2

2. Zhai J., Xiao L., Mo W., Long Y. The method for barcoded gel beads preparation and its application. 202010047034.1

3. Zhai J., Long Y., Jia J., Wen J., Lu D. The Preparation Methods and Applications of Novel Coronavirus-Like Particles. 202110333400.4