吴柘，2005年本科毕业于青岛大学。2011年博士毕业于北京大学生物技术专业，师从瞿礼嘉教授及顾红雅教授。2008-2009年作为访问博士生公派至日本京都大学学习。2011-2016年在英国约翰英纳斯中心，任博士后及高级博士后研究员，师从Caroline Dean爵士。2016年11月-2022年11月在南方科技大学生物系任助理教授，2022年12月通过国际评审晋升长聘副教授。2023年获得中国植物生理与分子生物学学会-卫志明青年创新奖，入选教育部人才计划。作为通讯作者在Nature Genetics, Genome Biology, Plant Cell, Molecular Plant, New Phytologist, Plant Methods等主流期刊上发表论文多篇。现任Science China-Life Sciences青年编委，Essays in Biochemistry 编委等。

吴柘实验室主要研究植物生殖的分子调控机理，重点探究RNA-蛋白在转录同时的包装过程以及表观遗传调控在其中所发挥的作用及机制。在方法上，我们采用遗传学及前沿的表观组学技术为研究的主要手段，结合重要的生物学过程，我们希望即见树木（单个重要的基因）又见森林（在基因组范围内的普遍模式和规律），揭示植物发育及基因表达调控背后的普遍规律。

研究领域

1. RNA结合蛋白在发育及环境响应中的作用范式

2. 转录及其偶联的RNA命运决定机制

3. 种子形成前后表观遗传/RNA调控对发育程序及发育稳健性的作用机制

研究成果

2025

 27. Wu, Z.# PRC2-mediated repression of PIF4 targets beyond the on/off switch of gene expression.(2025). Developmental Cell. 60:2025-2026

 26. Jang, G., Payne-Dwyer, A., Maple, R. Wu, Z., Liu, F., Lopez, S., Wang, Y., Fang, X., Leake, M., Dean, C. Modular in vivo assembly of Arabidopsis FCA oligomers into condensates competent for RNA 3’ processing. (2025) EMBO Journal 44: 2056 - 2074

2024

25. Menon, G., Mateo-Bonmati, E., Reeck, S., Maple, R., Zhe, W., Ietswaart, R., Dean, C., Howard, M.  Proximal termination generates a transcriptional state that determines the rate of establishment of Polycomb silencing. (2024). Mol cell.  84:2255-2271.

 24. Zhu, D#., Wen, Y., Tan Y., Chen, X.,# Wu, Z.#  A simple, robust, cost-effective, and low-input ChIP-seq method for profiling histone modifications and Pol II in plants.(2024). New phytologist.  244:1658-1669.

2023

23. Zhu, D., Wen, Y., Yao, W., Zheng, H., Zhou, S., Zhang, Q., Qu, L.J., Chen, X.,#and Wu, Z.# (2023). Distinct chromatin signatures in the Arabidopsis male gametophyte. Nature Genetics. 55:706-720. (co-corresponding author)

22. Zhou, S., Zhao, F., Zhu, D., Zhang Q., Dai Z., Wu Z.# (2023). Coupling of co-transcriptional splicing and 3′ end Pol II pausing pausing during termination in Arabidopsis. Genome Biology. 204:206. (co-corresponding author)

21. Chen L., Xu Z., Huang J., Shu, H., Hui, Y., Zhu, D., Wu, Y., Dong, S., Wu, Z.# Plant immunity suppressor SKPR encodes a novel RNA-binding protein that targets exon 3′ end of unspliced RNA. New Phytologist. 240:1467-1483.(co-corresponding author)

2022

20.  Zhang Q, Zhao F, Wu Z, Zhu D. A simple and robust method for isolating and analyzing chromatin-bound RNAs in Arabidopsis. Plant Methods. 2022, 18:135.

19. Yang D, Zhao F, Zhu D, Chen X, Kong X, Wu Y, Chen M, Du J, Qu L,-J, Wu, Z#. Progressive chromatin silencing of ABA biosynthesis genes permits seed germination in Arabidopsis. Plant Cell 2022, 34:2871-2891.

18.  Tong J, Ren Z, Sun L, Zhou S, Yuan W, Hui Y, Ci D, Wang W, Fan LM, Wu Z, Qian W. ALBA proteins confer thermotolerance through stabilizing HSF messenger RNAs in cytoplasmic granules. Nat Plants 2022, 8:778-791.

17.  Shang L, Li X, He H, Yuan Q, Song Y, Wei Z, Lin H, Hu M, Zhao F, Zhang C, et al: A super pan-genomic landscape of rice. Cell Res 2022.

2021

16. Xu, C*., Wu, Z*., Duan, H.C*., Fang, X., Jia, G., and Dean, C. (2021). R-loop resolution promotes co-transcriptional chromatin silencing. Nat Commun 12, 1790.

15.  Wang, Q., Xue, Y., Zhang, L., Zhong, Z., Feng, S., Wang, C., Xiao, L., Yang, Z., Harris, C.J., Wu, Z., et al. (2021). Mechanism of siRNA production by a plant Dicer-RNA complex in dicing-competent conformation. Science 374, 1152-1157.

14.  Zhao, N., Su, X.M., Liu, Z.W., Zhou, J.X., Su, Y.N., Cai, X.W., Chen, L., Wu, Z., and He, X.J. (2021). The RNA recognition motif-containing protein UBA2c prevents early flowering by promoting transcription of the flowering repressor FLM in Arabidopsis. New Phytol.

13.  Xu, Z., Shi, X., Bao, M., Song, X., Zhang, Y., Wang, H., Xie, H., Mao, F., Wang, S., Jin, H., et al. (2021). Transcriptome-Wide Analysis of RNA m(6)A Methylation and Gene Expression Changes Among Two Arabidopsis Ecotypes and Their Reciprocal Hybrids. Front Plant Sci 12, 685189. 

2020

12.  Wu Z#.  (2020): An introduction to the themed issue on RNA biology in China. Essays in Biochemistry, 64, 863-866.

11.  Zhu D*., Mao F*., Tian Y., Lin X., Gu L., Gu H., Qu L.J., Wu Y#., and Wu Z#. (2020): The features and regulation of co-transcriptional splicing in Arabidopsis. Molecular Plant, 13, 278-294.

10.  Wu Z., Fang X., Zhu D., Dean C. (2020). Autonomous pathway: FLC repression through an antisense-mediated chromatin silencing mechanism. Plant Physiology 182, 27-37.

9.  Fang XF*., Wu Z*., Raitskin O, Webb K., Voigt P., Lu T., Howard M., and Dean C. (2020). The 3′ processing of antisense RNAs physically links to chromatin-based transcriptional control. PNAS, 117, 15316-15321.

Before 2017

8.  Huang J., Gu L. Zhang Y., Yan T., Kong G., Kong L., Guo B., Qiu M., Wang Y., Jing M., Xing W., Ye W., Wu Z., Zhang Z., Zheng X., Gijzen M., Wang Y., Dong S. (2017). An oomycete plant pathogen reprograms host pre-mRNA splicing to subvert immunity. Nature Communications 8, 2051-2063.

7.  Ietswaart, R.*, Rosa, S.*, Wu, Z.*, Dean, C., and Howard, M. (2017). Cell-size-dependent transcription of FLC and its antisense long non-coding RNA COOLAIR explain cell-to-cell expression variation. Cell Systems 4, 622-635 e629.

6.  Wu, Z.*, Ietswaart, R.*, Liu, F., Yang, H., Howard, M., and Dean, C. (2016). Quantitativeregulation of FLC via coordinated transcriptional initiation and elongation. PNAS 113,218-223.

5.  Wu, Z.*, Zhu, D.*, Lin, X.*, Miao, J.*, Gu, L., Deng, X., Yang, Q., Sun, K., Cao, X., Tsuge, T., Dean, C., Aoyama, T., Gu, H., and Qu, L.J. (2016). RNA-binding proteins RZ-1B andRZ-1C play critical roles in regulating pre-mRNA splicing and gene expression duringdevelopment in Arabidopsis. The Plant Cell 28, 55-73.

4.  Wang, Z.W.*, Wu, Z.*, Raitskin, O., Sun, Q., and Dean, C. (2014). Antisense-mediated FLCtranscriptional repression requires the P-TEFb transcription elongation factor. PNAS 111,7468-7473.

3.  Zhu, D.*, Wu, Z.*, Cao, G.*, Li, J., Wei, J., Tsuge, T., Gu, H., Aoyama, T., and Qu, L.J. (2014). TRANSLUCENT GREEN, an ERF family transcription factor, controls water balance in Arabidopsis by activating the expression of aquaporin genes. Molecular Plant 7,601-615.

2.  Marquardt, S., Raitskin, O., Wu, Z., Liu, F., Sun, Q., and Dean, C. (2014). Functionalconsequences of splicing of the antisense transcript COOLAIR on FLC transcription.Molecular Cell 54, 156-165. 

1.  Ietswaart, R., Wu, Z., and Dean, C. (2012). Flowering time control: another window to the connection between antisense RNA and chromatin. Trends in Genetics 28, 445-453.